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Specifically, live cells were labeled with 2 M Calcein AM solution prepared in PBS (pH 7.4), while dead cells were identified using 4.5 M PI solution

Khodir, M.A

In conclusion, the study robustly demonstrated that FOXO4-DRI selectively eliminates senescent Leydig cells, reduces detrimental SASP factor secretion, improves the testicular microenvironment, and ultimately enhances sperm quality and spermatogenesis in aged mice, positioning it as a promising therapeutic candidate for age-related male infertility [2]

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Zeisel, A