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7-Dichlorofluorescin Diacetate (DCFDA) Assay for Production of Reactive Oxygen Species Cells were incubated in 10 M DCFDA (Sigma) in PBS at 37C for 30 min as previously described (Chiu et al., Quantification of 8-Hydroxy-2-Deoxyguanosine (8-OH-dG) as a Marker for Oxidative DNA Damage We used 8-Hydroxy-2-Deoxyguanosine (8-OH-dG) as a biomarker for oxidative DNA on brain cells exposed to different treatments using the commercially available HT 8-OH-dG ELISA II kit (R&D Systems) as guided by the manufacturers instructions

Citation on PubMed Ferenci P

Non-small-cell lung carcinoma tumor growth without morphological evidence of neo-angiogenesis

In mice and rats, tirzepatide caused a dose-dependent and treatment-duration-dependent increase in the incidence of thyroid C-cell tumors (adenomas and carcinomas) after lifetime exposure at clinically relevant plasma exposures

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Based on these encouraging results from the phase II study and following consultation with the European Medicines Agency (EMA), we then established a multi-centre phase III parallel arm 6-month study of Efmody versus standard therapy followed by an open-label Efmody extension safety study [16]