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The N-terminus and His-13 are crucial for Cu 2+ binding, and His-6 and His-14 are also implicated

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For enzyme-coupled assays, 50 U alkaline phosphatase FastAP (EF0651, Thermofisher Scientific, Waltham, Massachusetts, United States), 515 U glutathione reductase (G3664-100UN, Sigma Aldrich, St

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