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ROS is a broad term that includes oxygen radical and non-radical compounds, such as H2O2, hydroxyl radicals, lipid radicals and (phospho)lipid hydroperoxides (82)

ROS measurements For 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA, Sigma-Aldrich #D6883) and pentafluorobenzenesulfonyl fluorescein (PBSF, CaymanChem #728912-45-6) assays, 4 10 4 HCT116 cells were seeded per well in 24-well plates and allowed to adhere overnight

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137,138 This diversity in surface markers and cytokines produced by Bregs may be attributed to inflammatory signals specific to the local environment along with other stimuli, such as TLR agonists and metabolites derived from the microbiota