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Global protein abundance profiling by mass spectrometry Sample preparation (A) Flash frozen liver sections (~25 mg) from WT or Gclc / mice (3 biological replicates each per TMT sample) were thawed on ice and resuspended in DPBS, supplemented with protease (cOmplete, EDTA-free protease inhibitor cocktail, Roche, #11873580001) and phosphatase (PhosSTOP, Roche, #4906845001) inhibitor tablets

doi: 10.3389/fendo.2024.1503905 Received 29 September 2024 Accepted 09 December 2024 Published 09 January 2025 Volume 15 - 2024 Edited by Weibin Bai, Jinan University, China Reviewed by Zia Ur Rehman, University of Agriculture, Pakistan Kamil Gill, Pomeranian Medical University, Poland Updates Copyright 2025 Saleh, Sallam, Elsuity, Dutta, Sengupta and Nasr

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