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NHEK cells were maintained in Keratinocyte Basal Medium 2 (KBM2, PromoCell) supplemented with 0.4% bovine pituitary extract, 0.125 ng ml 1 rh EGF, 5 g ml 1 rh insulin, 0.33 g ml 1 hydrocortisone, 0.39 g ml 1 epinephrine, 10 g ml 1 transferrin and 0.06 mM CaCl 2

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Freezing and thawing a reconstituted peptide can cause the formation of ice crystals that, on a microscopic level, can shear and degrade the peptide chains