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are devoid of all granulocytes,28 and mice congenitally deficient in PU.1 are unable to generate terminally differentiated eosinophils.4 As may be predicted, many of these transcription factors are important in generating eosinophil lineage-specific granule proteins, such as major basic protein (MBP)-1, along with CCR3 and the subunit of IL-5R.29 For progenitor cells to become committed to eosinophil development requires concomitant expression of C/EBP, PU.1, and low-to-moderate GATA-1, with no expression of FOG-1

It is important to note that this amplified response, due to the delayed production of the secondary proteins, may occur in either the relative absence of the activating hormone or alternatively at the time that the steroid receptor is apparently down regulated

eLife 6:e28083 [DOI] [PMC free article] [PubMed] [Google Scholar] Schofield CJ, Ratcliffe PJ (2004) Oxygen sensing by HIF hydroxylases

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Tube formation in sandwich culture was performed as described previously (8) with slight modifications