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Nat Cell Biol (2012) 14(3):27686

Dissociation constants of polybasic acids and their application to the calculation of molecular dimensions

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The diminished protein thiol content, susceptibility to perturbations in methionine metabolism, evidence of chronic low-grade hepatic pathology and occasional spontaneous death in mice with TR/GR-null livers suggest that homeostasis in these animals is at the brink of failure

These changes increase the half-life beyond 30 minutes which is more than sufficient to exert a sustained effect which will maximize a GH pulse