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This reagent provides a colorimetric readout when it binds to exposed free thiols, thereby detecting the lipoate moiety if an adduct is removed (Fig

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AghdamMSLuoZJannatizadehASheikh-AssadiMSharafiYFarmaniBet al

In addition to NL of 129 Da, other unique fragmentation pathways of GSH adducts such as NL of 307 Da or formation of product ion at m/z 149 can be applied as filters to process the EPI data file, which may provide better selectivity and sensitivity than NL filtering of 129 Da in finding certain types of GSH adducts [27, 33]

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Further investigations revealed that mammalian lipoxygenase family member arachidonate 12-Lipoxygenase (ALOX12) is crucial for p53-dependent ferroptosis