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The peak fractions were concentrated on a 10 kDa MWCO Amicon concentrator and applied onto a Superose 6 10/300 column (Cytiva) pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 1 mM TCEP)

[DOI] [PubMed] [Google Scholar] 180.Patterson JE, Weissberg BG, Dennison PJ

Int J Herb Med 4(3):2127 Chandel NS, Maltepe E, Goldwasser E, Mathieu CE, Simon MC, Schumacker PT (1998) Mitochondrial reactive oxygen species trigger hypoxia-induced transcription