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GHK-Cu downregulates acute-phase and pro-inflammatory cytokines (TNF-?, IL-6), upregulates anti-oxidant defenses (including superoxide dismutase, Nrf2 and HO-1 pathways), and suppresses fibrotic signaling by inhibiting TGF-?1/Smad2/3 activation

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Required Reagents and Equipment PBS pH 7.6 DMSO Low speed centrifuge used for cell culture Rocker table All biotinylation steps should be performed at room temperature

1996) Two thiyl radicals can combine and form a non-radical oxidized glutathione (GSSG) The GSH/GSSG ratio is a good marker of oxidative stress of an organism (Hwang et al