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Aliquots (3 L) of ethanolic cell pellet extracts were analysed by means of UPLC-TOF MS using the following solvent gradient for chromatography (0.4 mL/min, 45 C): starting with a mixture (10/90, v/v) of water and methanol, the methanol content was increased to 100% within 8 min and, then, kept constant for 1 min

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This capability to generate a beneficial microenvironment is essential for tissue regeneration because it reduces excessive inflammation, which can delay the recovery process (Bjrk et al., 2024)

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FlagCTPS1 was then combined with pre-warmed (37 C) nucleotides at final concentrations of 2 mM UTP, 2 mM ATP and 0.2 mM GTP for 30 min