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CL4B resins were pelleted at 16,900 g and 4 C for 1 min, and the supernatants were then incubated with 200 l M2 magnetic beads (Sigma-Aldrich, M8823) and immunoprecipitated at 4 C for 16 h

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Table 4 Current administration methods of hydrogen and their advantages and disadvantages

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Taking a glimpse at autophagy, SIRT1 positively enhances the autophagic process through the deacetylation of many proteins, among which are Beclin-1 and autophagic-related genes such as ATG5 (Sacitharan et al

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