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Quantitative real-time PCR (QRT-PCR) was performed using the One Step SYBR Ex Taq qRT-PCR Kit (TaKaRa Bio inc, Japan) on a Rotor-Gene 6000 instrument (Corbett Research, Australia) under the following cycling conditions: initial denaturation at 95 C for 30 sec, followed by 40 cycles of 95 C for 30 sec and 60 C for 30 sec

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FIGURE 3 The role of residues present in the immediate vicinity of the cleavage site during autoprocessing was also explored