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Chemicals Unless otherwise indicated, the chemicals, culture medium, and supplements used in this study were purchased from Sigma-Aldrich (Shanghai, China) and Thermo Fisher (Beijing, China)

Another study found that glutathione inhibits melanin formation by direct inactivation of the enzyme tyrosinase by binding and chelating copper within the enzymes active site

Daily use helps maintain stable glutathione levels, especially in the face of constant exposure to pollution and stress

Sucrose preference test For clarity and adherence to the experimental timeline, the sucrose preference test was performed after these behavioral tests to assess the impact of PCPA-induced insomnia on sucrose preference in the animals

Cobalt sensitivity reactions can result in different symptoms, such as chronic or subacute allergic contact dermatitis, which can occur immediately or up to 72 hours after administration, but the likelihood of a cobalt allergy is low and affects between 1% and 3% of the general population