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Kster et al
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For subsequent deubiquitinase assay, ANKIB1 was removed with M2-Flag beads and either USP2 or Cezanne were added at a concentration of 1 M and 0.5 M, respectively, and samples were incubated at 30 C for 1 h
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Remove air bubbles by holding the syringe needle-up and tapping gently
