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However, this method has its disadvantages, including the use of a somewhat arbitrary safety factor to ensure safety of the starting dose, the dose is based on minimal risk of toxicity rather than based on pharmacologic activity, and it does not address dose escalation or the maximum allowable dose

Corroon J, Phillips JA

To identify the SSR marker in MtGSTs , microsatellite identification tool (MISA, [44] was used with at least ten units for mononucleotide repeats and a minimum five units for dinucleotide, trinucleotide, tetranucleotide, pentanucleotide, and hexanucleotide repeats

6.1 Challenges However, current research faces several critical challenges