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We found that about 2,000 genes are differentially regulated (false discovery rate less than 5% and log-2 fold change larger than 1) in at least one mutant cell line compared to parental SW48 cells (Figures 1A and S1E-F)

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Site-directed mutagenesis, gene synthesis, and cloning Point mutations were introduced by PCR with Pfu polymerase (Promega) using the mutagenesis primers listed in Supplementary Table 10 and the double stop-codon construct of pQE30/ SCGRX7 (ref