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However, already at low doses it appeared to influence control cells as well (Fig

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The high turnover rate and limited storage of thiamine (20 30 mg, mainly in the heart, brain, liver, and kidneys) require an adequate daily thiamine intake to meet requirements

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The rationale behind requiring a prescription for B12 injections stems from safety and regulatory concerns

After chondrocytes reached 8090% confluency, the cells were dissociated with Trypsin/EDTA (Gibco/Thermo Fisher Scientific) and passaged