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Representative tissue specimens (i.e., the brain, liver, kidney, pancreas, stomach, small and large intestine, lungs, and heart taken at the end of the experiment, fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h) were embedded in paraffin, sectioned at 4 m, and stained with hemalaun and eosin (H&E)

The resulting CRISPR/Cas9-PEI plasmid complexes were then incubated with 100 l of bare MNP (200 g/mL) in water for 1 h

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The drug dosage and number in each group are shown in Table 1

Intracellular pathways such as ERK and IRS2-AKT are highly regulated by stress and drugs of abuse and involved in regulation of mood-related disorders and drug-induced neuroplasticity 73,74

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