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Second, solvent was injected forcefully into the center of the lyophilized powder, creating visible foam

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It is known that CNS cultures involving serum can lead to reactive changes in CNS cells, especially astrocytes, as reported in previous studies comparing prospective astrocyte isolation (e.g., immunopanning or FACS-based isolation) to the classic culture and enrichment of astrocytes according to McCarthy and de Vellis (1980) (Fu et al., 2015

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