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This is because in the control, some nisin residues no longer form hydrogen bonds with water molecules, but they stay close enough to the water molecules to form contacts, i.e., the lost hydrogen bonds are now counted as contacts

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Many characteristics that vary within studies, such as the type of study, the year of publication, the inclusion and exclusion criteria, the sample size, the control group or quality of the studies, could have been the factors of between-study heterogeneity

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The makers of Coolsculpting put together animation to show how the process works