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The precipitate was collected (6000 rpm, 30 min, 4 C) and washed with 1 mL of acetone three times to remove the TCA

Indeed, some studies have shown that collagen hydrolysate can boost the synthesis of collagen by skin fibroblasts

J., Herzenberg, L

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Probe 1426 in the presence of Ampligase (Lucigen) was used to selectively circularize and protect fragments containing the barcoded transposon sequence, while all other fragments were digested with a mix of T7 gene 6 exonuclease (ThermoFisher), Lambda exonuclease (NEB), and Exonuclease I (NEB)

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