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Except the positive control using in the kit, another two cases of Langerhans cell histiocytosis (LCH) which were previously confirmed with BRAF V600E mutation were also used as a positive control

However, the latter was not predictive of H3K27me3 immunohistochemical staining, so H3K27me3 expression may be more accurate in this context

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Therapeutic effects of melatonin receptor agonists on sleep and comorbid disorders