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RNA isolation and quantitative real-time PCR Total RNA was isolated by using TRIzol reagent (Invitrogen, Carlsbad, CA, USA)
& Thompson, R
J., Lafon, N., Pio, A., Rinaldi-Carmona, M., Le Fur, G., & Soubri, P
O., Larsen, F
Subsequently, TFAM translocates to the mitochondrial matrix, where it stimulates mtDNA replication and mitochondrial gene expression, but also act as protection against mtDNA damage and oxidation (Kang et al
Notably, controlled fluid flow introduces sustained perfusion and physiologically relevant shear forces, both of which modulate cellular behaviour and allow for a more accurate assessment of nanoparticle transport, distribution and cellular uptake than static in vitro models [175, 176]
