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DNA intrastrand cross-link at the 5-GA-3 sequence formed by busulfan and its role in the cytotoxic effect

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VPS35-BioID1 and BioID1-VPS35 proximity labelling, followed by targeted western analysis, confirmed the selective labelling of VAMP3, CAV1 and CAVIN1, and subunits of the augmin complex within the Retromer sub-domain, suggestive of these proteins and complexes associating with and/or transiently traversing through this sub-domain (Fig