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Initial assessment in rats with two ligations at the left colic artery and vein for 15 min (IC rats) and in rats that had two ligations at the left colic artery and vein for 15 min, and then were reperfused for the next 15 min (IC + RL rats) The surgery was conducted in deeply anesthetized rats

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[DOI] [PubMed] [Google Scholar] 111.Rani M., Aggarwal R., Vohra K

In case of a darker complexion or an uneven skin, there is also an elevated production of Eumelanin (a pigment responsible for imparting the dark brown color)