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[57] Approval of the original brand-name versions (such as Ozempic) was based on a biologic (recombinant DNA) production process using a genetically-modified yeast ( Saccharomyces cerevisiae ) to produce a precursor to semaglutide, and subsequent chemical processes (protein purification) to create the active ingredient

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A total of 6,098 participants were included (mean: 244)

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Flow cytometry Cell cycle analysis of cells cultured in liquid medium was performed as described [28] using a FACS Canto II flow cytometer (BD Bioscienes, San Jose, CA, USA)