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Like the majority of cancers, GBM has an uncommon aerobic glycolysis state that GBM preferably utilizes an aerobic glycolytic pathway rather than oxidative phosphorylation resulting in the production of lactate, known as the Warburg effect 19,20

Under oxidative stress, excessive ROS such as OH directly attack DNA, causing single-strand or double-strand breaks, activating PARP-1, consuming NAD and ATP, and generating poly(ADP-ribose), resulting in mitochondrial membrane potential loss and the release of apoptosis-inducing factor, ultimately resulting in cell death [252]

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Immunohistochemistry and immunofluorescence Liver tissues were collected from patients or mice, as mentioned above