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Importantly, although pre-existing humoral and cellular immunity to commonly used Cas orthologs (e.g., SpCas9/SaCas9) has been documented in human populations (295), published clinical experience with CRISPRCas9 therapies to date has not reported clinically meaningful Cas9-directed immune toxicities (296)

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[38] determined the effect of treatment with ALCAR on the functional impairment of mitochondria from brain at 20 hours after HI

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These findings provide a basis for the timely adjustment of personalized treatment strategies, driving the advancement of tumor therapy toward dynamic and precise regulation