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Advanced Analytical Verification: Every batch is validated through HPLC and Mass Spectrometry (MS) to confirm chemical identity and ensure precise acylation positioning

doi: 10.1016/j.ccell.2017.11.015

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HgCl 2 Parameters Organs Control LD HD Melatonin Melatonin + HD Anova Amelioration (%) CH 0.0058 0.0003 0.00594 0.0002 NS 0.00658 0.0002 * 0.00592 0.0002 NS 0.00612 0.0001 NS 0.82 # 54.28 C 0.0035 0.0001 0.0044 0.0001 + 0.00545 0.0002 + 0.00352 0.0002 NS 0.0120 0.0012 NS 1.77 # 64.10 GST (units/mg protein) MO 0.00256 0.001 0.0027 0.0003 NS 0.00296 0.0001 * 0.00260 0.0001 NS 0.00280 0.0002 NS 4.19 # 40.00 CH 10.7 0.80 11.7 0.95 NS 12.9 0.97 * 09.7 0.50 NS 11.1 0.40 NS 1.85 # 81.80 C 09.3 0.70 10.0 0.29 NS 10.9 0.07 * 09.4 0.60 NS 10.2 0.13 NS 6.02 # 43.75 Protein Carbonyl (nmol/ mg protein) MO 10.2 0.33 10.7 0.30 NS 11.1 0.28 * 10.0 0.70 NS 10.8 0.30 NS 1.05 # 33.70 CH 69.6 5.64 77.2 5.31 NS 80.2 1.20 NS 64.0 5.02 NS 73.0 3.06 NS 1.68 # 67.90 C 55.2 4.70 58.8 1.74 NS 75.6 3.42 NS 54.4 3.48 NS 59.6 2.03 NS 2.18 # 78.43 H 2 O 2 (M of H 2 O 2 formed/100 mg tissue wt) MO 50.6 5.29 66.2 4.56 NS 74.4 3.61 NS 48.2 1.15 NS 58.4 4.16 NS 4.27 # 67.20 CH 2.10 0.17 1.59 0.09 * 1.47 0.05 ** 1.54 0.06 NS 1.06 0.12 ** 7.12 # 65.23 C 1.66 0.12 1.54 0.12 NS 1.22 0.11 * 1.68 0.1 NS 1.31 0.05 NS 2.69 # 20.45 Total (SH) (g/100 mg fresh tissue wt) MO 1.97 0.14 1.65 0.04 NS 1.42 0.17 * 2.01 0.01 NS 1.66 0.077 1.75 # 43.90 Values are Mean S.E., HgCl 2 = Mercuric Chloride

It should be noted that only interactions with reactive species that resulted in a chemical reaction are depicted in both figures