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For example, in wheat, BSMV-based VIGS has been utilized to mute resistance genes like Lr21 as well as stress-related genes like HSP90, RAR1, and SGT1, by overcoming the limitations of the polyploid genome [12]

PMID: 10642559

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To generate double-stranded DNA probes, oligonucleotides were annealed by heating at 95 C for 5 min, followed by slow cooling to room temperature

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