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Baseline measurements were taken after an initial 5 min incubation with coelenterazine-h or NanoGlo-containing HBSS, after which cells were treated with either a vehicle (PBS) or the respective ligands

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Similarly, the number of viable cells after the addition of 0.1 g/mL growth hormone to BPC 157-treated cells for one to three days significantly increased in a time-dependent manner (Figure 2B), proving that BPC 157 pretreatment could potentiate the effect of growth hormone on increasing the number of viable cells
