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The cells were lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Calbiochem, 524-624 and 524-625) and subsequently subjected to western blotting using the indicated antibodies as described previously 16

Transplant International, 7 (3), 190194

Similarly, individuals who engaged in walking or biking for over 60 min daily recorded a 12% lowered prevalence of cataracts in comparison to those who rarely partook in such activities (49)

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