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Cancer treatments significantly elevate OS through multiple mechanisms: chemotherapeutic agents, particularly alkylating agents and platinum compounds, generate excessive ROS directly damaging sperm DNA and membranes (87, 88), while radiation therapy induces both acute and chronic OS states in testicular tissue, leading to prolonged spermatogenic dysfunction (95)

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Radiation-induced cell death and its mechanisms

Nat Genet 49, 367376

This is in stark contrast to the original purple hue observed at the 0 hour and 1 hour trials within Fig