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The cultures were inoculated to an OD 600 of 0.1 from precultures that were made in standard M9 medium supplemented with 1% (v/v) LB medium and placed in a shaking incubator (37C, 180 min 1 shaking frequency, 25 mm eccentricity)

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It is synthesised naturally from the amino acids lysine and methionine, primarily in the liver and kidneys, though it can also be obtained through dietary sources such as red meat and dairy products

These individuals should avoid alcohol, and purine rich foods such as red meats, organ meats, and legumes

That said, some people report mild stomach upset, especially at higher doses