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The Nissl-stained slides were observed using a Nikon microscope

Recent work has established that the heterogenous nuclear ribonucleoprotein hnRNPA2/B1, a ubiquitous RNA binding protein involved in RNA processing, is a negative regulator of ATP7A expression and has implicated this RNA binding protein in the developmental expression of ATP7A (McCann, Hasan, et al., 2022)

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To address these limitations, the current protocol employs a Tiron/ferrous ion (Fe 2+ ) reagent, which eliminates the requirement for protein precipitation and effectively resolves the issues associated with earlier methods

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Moreover, GSH levels also serve as a prognostic marker of the malignancy of brain cancer