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Introduction GPCRs, as the largest membrane protein superfamily, are categorized into five distinct subfamilies: the rhodopsin-like family (Class A), the secretin/adhesion family (Class B), the metabotropic family (Class C), the smoothened/frizzled family (Class F), and the taste2 family (Class T)
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Local ice application typically lasts 15-20 minutes per session, limited to prevent tissue damage from prolonged cold exposure
