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1.9 nmol of protein in a total volume of 1 mL was applied to a Resource RPC column with 1 mL bed volume (Cytiva, Freiburg, Germany) equilibrated with buffer A (2 % (v/v) acetonitrile, 0.1 % (v/v) formic acid)

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Nat Rev Microbiol 2019, 17 (3), 167180

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