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Sources: Chang CH, et al

[DOI] [PMC free article] [PubMed] [Google Scholar] 170.Volpicelli-Daley L.A., Abdelmotilib H., Liu Z., Stoyka L., Daher J.P.L., Milnerwood A.J., Unni V.K., Hirst W.D., Yue Z., Zhao H.T., et al

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Alexa Fluor conjugated secondary antibodies were used to fluorescently label the antigens of interest and following dehydration in a series of increasing concentrations of methanol and cleared using dibenzyl ether, the membrane-bound slices were placed onto a glass microscope slide and imaged using a Zeiss LSM-800 inverted confocal microscope

CLC transplantation has its limitations because these cells can only be obtained from herniated discs, cell numbers are low, and extensive expansion before transplantation is needed [32]
