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These symptoms indicate gut microbiome shifts beginning

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Fluorescence was measured at 4, 24, 48 and 72 h after treatment at 485 nm excitation and 530 nm emission on a SpectraMax M3 microplate reader (Molecular Devices)

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Tensiometry The investigation in separate groups of animals, includes measurement of ability to stretch (mm) carried out in separate experiments, immediately after sacrifice, at days 7, 14, 21, 42 by a special device Lineomat (MLW Medizinische Gerte, Chemnitz, Germany)

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