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Collected fractions were run on SDS-PAGE to verify protein expression and dialyzed into storage buffer (25 mM Tris [pH 8.6], 20% glycerol, 100 mM NaCl, 1 mM DTT)

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The finding of Ileibacterium as the most abundant cecal microbiome genus in every experimental group is relatively novel and may reflect experimental factors such as diet, housing conditions, and mouse strain
Vitamin B12 Correlations with Sleep Quality, Daytime Sleepiness, and Insomnia Symptoms Table 2 and Figure 2 demonstrate differences in questionnaire scores between people with low vitamin B12 levels and those without
