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Afterwards, tubes were briefly centrifuged and real-time PCR was carried out in a 7500 Real-Time PCR System (Applied Biosystems, Foster, CA, USA) using the following cycling parameters: 2 min at 50C
Higashi, Y., Strominger, J.L
Acetylcholine Synthesis : The acetyl group from ALCAR can be donated to choline to form acetylcholine, a key neurotransmitter involved in memory formation and cognitive processing
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doi: 10.1161/ATVBAHA.107.160192
The fitting routine did not always fit all the components in all the spectra, which is reflected by the number of participants for a given metabolite in Table 2
