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Description
scFv TS2/16 production in Expi293 cells The coding sequence of the TS2/16 scFv (V H V L orientation) containing the artificial human kappa L-chain signal sequence, and C-terminal tags (8His tag), HRV 3C site (LEVLFQ/GP), Fc-tag (huIgG1) and a stop codon, was cloned into the pcDNA3.1 mammalian expression vector (Extended Data Fig

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J Biol Chem 277, 4814648151, doi: 10.1074/jbc.M209165200 (2002)

The cDNA was amplified using KOD Xtreme Hot Start DNA 396 Polymerase (Millipore Sigma) flanking the S encoding sequences

Piec, P.-A
The project described was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grants K12TR004416 and U01TR003715
