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The combined effect of furosemide and propranolol on GSH homeostasis in ACHN renal cells

To analyse reduced and oxidized PDI by western blot, cells were lysed in 100 mM Tris-HCl pH 6.8, 2% SDS supplemented with PhosSTOP and cOmplete protease inhibitor cocktail and 40 mM ethylmaleimide to block free cysteines

Zhu F, Gujar H, Gordon JR, Haynes KF, Potter MF, Palli SR

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First, hydrochloric acid in the stomach separates vitamin B12 from the attached protein