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Instead, dilute 10 l into 90 l of assay buffer, then perform a subsequent 100-fold dilution by adding 10 l into 990 l of assay buffer

Intermittent fasting alleviates risk markers in a murine model of ulcerative colitis by modulating the gut microbiome and metabolome

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Fully differentiated cells were transfected with control or GPR146 siRNA, and 48 h after transfection, mitochondrial respiration was assessed using the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies) according to the manufacturers instructions