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After 24 h postplating time, fresh media were supplied (200 l/well) and treated with either an equal volume of sterile water (vehicle control) or with 23.4 mmol/l of ICA-1s

If you reconstitute a 30mg vial at 10mg/mL and your weekly dose is 2.5mg, you have 12 weeks of product in that vial

reconstituted material is refrigerated and used within a limited window