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Unresolved questions include the tissue-specific regulation of ULK1 complexes in PCa autophagy, role of CPS1 in nucleotide metabolism, and spatiotemporal dynamics of ATF6-mediated therapy resistance, all critical for advancing precision therapies in this metabolic-death nexus

For estimation of mitochondrial mass, the fly brains were stained with 20 M N-Nonyl Acridine Orange for 15 min and fluorescence intensity was determined as above

This forms a complete regulatory cascade:NNMT inhibition NAD+ elevation metabolic optimization This unique mechanism makes 5-amino-1-methylquinolinium injection a critical tool linking NNMT activity, nicotinamide metabolism, and systemic metabolic homeostasis, providing direct experimental support for exploring NNMT's role in energy metabolism