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Second, knockout of alsin in mice is not sufficient to trigger motor neuron degeneration, but neurons cultured from these animals are more susceptible to oxidative stress (Cai et al., 2005)
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cereus where the sortase D enzyme helps to polymerize the BcpA protein via two recognition signals, the LPXTG as the cleavage and thioester forming point, and the YPKN site which acts as the recognition signal as where the isopeptide will form

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However, its use is limited by the need for special training to perform the test