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Meanwhile, a culture of EcN pSIM18 was grown to mid-log phase at 30 C in LB + 200 g/mL hygromycin, then expression of the lambda Red proteins was induced by incubating the cells at 42 C for 15 min, followed by 15 min on ice

(2014) Data file S1 or Data files S1 to Sx (Excel files) References ( 11 ) is cited in Supp Material Funding This article was supported by the MoU signed between Inserm and the Mailman School of Public Health of Columbia University on Nov

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[DOI] [PubMed] [Google Scholar] 98.Serreli G., Deiana M